GLANDERS. 



453 



side of the track of the needle ; on glycerine-agar vsdth milk a 

 whitish layer develops, which gradually changes in colour from 

 amber yellow to a reddish-brown. On blood serum the growth is 

 transparent and yellowish ; on potato it is much more characteristic. 

 After two or three days at the temperature of the blood, a film 

 develops in the vicinity of the inoculated area, which is honey-like, 

 transparent and yellow ; the transparency disappears, and in a 

 week the cultures have become reddish-brown. (Plate II., Fig. 6.) 



The disease has been communicated to man by accidental 

 inoculation with a hypodermic syringe which had been used for 

 inoculating pultures. Horses, asses, cats, goats, field mice, and 



Fig. 185.— Section of a beanch oj? the Pulmonaey Aetbky showikg 

 G-LANDBRS Bacilli -PENETRATING THE Wall (Hamilton). 



guinea-pigs, can all be infected with pure cultures ; rabbits, sheep, 

 and dogs are slightly susceptible; cattle, swine, and white mice 

 have an immunity. In the guinea-pig a swelling occurs at the seat 

 of inoculation, followed by the formation of a prominent tumour, 

 developing into an abscess. The skin becomes involved, and an ul6er 

 with indurated margin results. The lymphatic glands also become 

 impHcated, and general; infection follows, extending frequently to 

 the testicles or ovaries, and death results after several weeks. In 

 rabbits there is generally only a local abscess induced, which termi- 

 nates in a quickly-heahng sore. Mice die in three or four days 

 from general infection ; glanders nodules are found in the liver and 



