398 Pierre A. Fish 



Max Flesch'^ recommends the addition of one part of cor- 

 rosive sublimate to three thousand parts of glycerin. A hu- 

 man brain he leaves in water for two days in order to wash 

 out the blood, it is then placed in alcohol for four weeks; then 

 for two weeks in equal parts of glycerin and alcohol and 

 finally four weeks in pure glycerin, to every three thousand 

 parts of which is added one part of corrosive sublimate (the 

 sublimate is dissolved in a small quantity of water and alcohol 

 and then added to the glycerin). Wherever it is necessary the 

 brain is supported upon a layer of cotton to avoid deformity. 



After the drainage of the superfluous glycerin the specimen 

 is again placed for final storage upon a piece of blotting paper 

 supported by a layer of cotton and the whole enclosed by a 

 paste board box with a glass top, to protect from the dust. 

 The expense is slight as the solutions can be used repeatedly. 

 The alba and cinerea are said to remain well differentiated. 



Struthers^" hardens the brain in alcohol after the removal of 

 the membranes, for ten or fourteen days. It is then put into : 



Glycerin 4 parts. 



Carbolic Acid i part. 



for two or three days. When the superfluous gl3'cerin drains 

 off, the brain is put under a glass case in order that it may not 

 take the dust. It is claimed that there is less shrinkage and 

 more flexibility than in Giacomini's method. 



Richardson'" recommends the following formula : 



Glycerin 300 c. c. 



Methylated spirit 600 c. c. 



Zincchlorid 2 grams. 



" Dissolve the zinc chlorid in the spirit and gradually add the 

 glycerin. In use immerse the structure in the solution and 

 keep it in until it is fully saturated. Then remove and let 

 harden," [dry]. 



As a result of numerous experiments and a careful study of 

 previous methods, the following process was devised : The 

 preliminary treatment is as directed on page 393. After dehy- 



