BACTERIOLOGICAL TECHNIC 



109 



Orientation (grouping) 



Agar J Chains (No. of elements) 



Hanging-block Short chains, long chains. 



Orientation of chains, parallel, irregular. 



2. Sporangia, medium used temp age days 



Form, elliptical, short rods, spindled, clavate, drum-sticks. 

 Limits of Size Size of Majority 



(Orientation (grouping) 

 Chains (No. of elements) 

 Orientation of Chains, parallel, irregular. 

 Location of Endospores, central, polar. 



3. Endospores. 



Form, round, elliptical, elongated. 



Limits of Size 



Size of Majority 



Wall, thiclt, thin. 



Sporangium wall, adherent, not adherent. 



Germination, equatorial, oblique, polar, bipolar, by stretching. 



4. Plagella, No Attachment, polar, bipolar, peritrichiate. How Stained 



5. Capsules, present on 



6. Zooglea, Pseudozooglea. 



7. Involution Forms, on in days at °C. 



8. Staining Reactions. 



1:10 watery fuchsin, gentian-violet, carbol-fuchsin. Loeffler's alkaline methylene 



blue. 

 Special Stains 



Gram Glycogen 



Fat Acid-fast 



Neisser. 



II. CULTURAL FEATURES (3) 



1. Agar Stroke. 



Growth, invisible, scanty, moderate, abundant. 



Form of growth, filiform, echinulate, beaded, spreading, plumose, arborescent, rhizoid. 



Elevation of growth, flat, effuse, raised, convex. 



Luster, glistening, dull, cretaceous. 



Topography, smooth, contoured, rugose, verrucose. 



Optical Characters, opaque, translucent, opalescent, iridescent. 



Chromogenesis (8) 



Odor, absent, decided, resembling 



Consistency, slimy, buytrous, viscid, membranous, coriaceous, brittle. 

 Medium grayed, browned, reddened, blued, greened. 



2. Potato. 



Growth, scanty, moderate, abundant, transient, peristent. 



Form of growth, filiform, echinulate, beaded, spreading, plumose, arborescent, rhizoid. 



Elevation of growth, flat, effuse, raised, convex. 



Luster, glistening, dull, cretaceous. 



Topography, smooth, contoured, rugose, verrucose. 



Chromogenesis (8) Pigment in water insoluble, soluble; other 



solvents 



