112 



PHARMACEUTICAL BACTERIOLOGY 



Presence of nitrites ammonia 



Presence of nitrates free nitrogen 



4. Indol production, feeble, moderate, strong. 

 S- Toleration of Acids: Great, medium, slight. 



Acids tested. 



6. Toleration of NaOH: Great, medium, slight. 



7. Optimum reaction for growth in bouillon, stated in terms of Fuller's scale 



8. Vitality on culture media: Brief, moderate, long. 



9. Temperature relations: 



Thermal death-point (10 minutes' exposure in nutrient broth when this is adapted 

 to growth of organism) C. 



Optimum temperature for growth C. : or best growth at 15° C, 20° C, 25° 



C, 30° C, 37° C, 4°° C, So° C, 60° C. 



Maximum temperature for growth C. 



Minimum temperature for growth C. 



10. Killed readily by drying: resistant to drying. 



11. Per cent, killed by freezing (salt and crushed ice or liquid air) 



12. Sunlight: Exposure on ice in thinly sown agar plates, one-half plate'covered (times 



15 minutes), sensitive, not sensitive. 

 Per cent, killed 



13. Acids produced 



14. Alkalies produced 



IS- Alcohols 



16. Ferments: Pepsin, trypsin, diastase, invertase, pectase, cytase, tyrosinase, oxidase, per- 

 oxidase, lipase, catalase, glucase, galactose, lab, etc 



17. Crystals formed 



18. Effect of germicides: 



Substance 



Method used 



Minutes 



Temper- 

 ature 



Killing 

 quantity 



Amt. required to 

 restrain growth 



IV. 'PATHOGENICITY. 



1. Pathogenic to animals. 



Insects, crustaceans, fishes, reptiles, birds, mice, rats, guinea-pigs, rabbits, dogs, 

 cats, sheep, goats, cattle, horses, monkeys, man. 



2. Pathogenic to Plants: 



3. Toxins, soluble, endotoxins. 



4. Non-toxin forming. 



5. Immunity bactericidal. 



6. Immunity non-bactericidal. 



7. Loss of virulence on culture-media: Prompt, gradual, not observed in months. 



