4l6 PHARMACEUTICAL BACTERIOLOGY 



XIV. Determining the Efficiency Value o£ Disinfectants. 



1. Phenol germ destroying coefficient. 



2. Toxic Coefficient. 



3. Albumen coagulating coefficient. 



4. Comparative cost. 



XV. Determining the Purity and Quality of Sera, Bacterins and of Related Products. 



1. Purity and freedom from bacteriological contamination. 



2. The purity of smallpox vaccines. 



3. Purity of bacterial vaccines. 



XVI. Special Biological and Toxicological Tests. 



1. Arsenic in foods. Biological test for arsenic. 



2. Toxicity tests with defibrinated blood, 

 (o) Toxalbumins and toxins. 



(i) Saponins. 



(f) Chemical hemolysis. 



3. Frog tests for the presence of alkaloids. 



The following blank will be found useful in making reports of 

 bacteriological examinations. In many instances however, it will be 

 found necessary to supplement the report or to make a special report. 



Form No. Ill 

 Bacteriological Examination 



(No., label, dates, condition of seals as for Form I.) 



I. Direct count. (Thoma-Zeiss hemacytometer with Turck ruling.) 



1 . Bacilli per cc 



2. Cocci per cc 



II. Plate and tube cultures. (Lactose-litmus-agar.) 

 T. Temperature differential test. 



(a) (20° C.) colonies per cc 



(*) (38° C.) colonies per cc 



2. Color differential test. 



(o) Pink or yellow colonies per cc 



(b) Not pink or yellow colonies per cc 



3. Gelatine liquefsdng colonies per cc 



4. Indol reaction (+) 



5. Neutral red reduction ( +) ; 



6. Gas (hydrogen) formula 



7. Gram-stain behavior (±). 



8. Presumptive colon bacillus test ( +), 



(a) Amounts used '..... 



(b) Number of tests 



(c) Rating '. 



III. Special tests 



IV. Conclusions. 



. Analyst. 



