4^4 APPENDIX. 



vulgaris all stain on the addition of larger or smaller portions of acid. With 

 alkali the bacillus crystallosus, micrococcus agilis, and the typhoid bacillus all 

 show flagella. The glanders bacillus, although said to be motile, has 

 apparently no flagella. 



Identification of Species of Bacteria. 



The Organism is a Micrococcus. 



The „ „ Bacillus, see p. 421. 



The „ „ Spirillum, see p. 438. 



The Organism is a Micrococcus. 

 I. The gelatine is not liquefied. 

 II. The „ is liquefied, see p. 418. 

 III. No growth on gelatine, see p. 421. 

 The gelatine is not liquefied. 



A. The colonies are white. 



B. The „ „ yellow, see p. 417. 

 c. The „ „ red, see p. 418. 



D. The „ ,, black, see p. 418. 



A. The colonies are white. 



a. The colonies are small, but confluent, growing slowly. 



b. Colonies confluent, growing luxuriantly, p. 416. 



a. The colonies are small, not confluent, growing slowly. 



(i) Streptococcus pyogenes. — On plates grow as small punctiform masses 

 J-mm. in diameter, at first appear white, pale yellow, and then brown, 

 under low power of microscope ; no tendency to run together in either 

 plate, puncture, or stroke cultivations, except on blood serum, or agar-agar, 

 where the mass is thicker in the centre ; terraced towards edges, and then 

 again discrete as in gelatine cultivations at the extreme margins ; no growth 

 on potatoes ; Cocci m. in diameter arranged in chains or diplococci ; not 

 pathogenic to mice or healthy rabbits ; frequently found in pus in human 

 subject and in lymphatics, near the spreading margin of a suppurating area. 



(2) Streptococcus erysipelatosus. — Very like the above, but diflers in that in 

 stroke cultivations the colonies have a somewhat greater tendency to run 

 together ; tViese appear whiter and more opaque, and have at the periphery 

 numerous outgrowths which consist of projecting chains, which give to the 

 cultivation the appearance of a fern-leaf ; found in the lymphatics of the 

 spreading zone of an erysipelatous area ; it sets up erysipelatous inflammation 

 when inoculated into the ear of a rabbit ; sets up typical erysipelas and not 

 suppuration in man. 



(3) Streptococcus pyogenes malignus. — Cultivated by Fliigge (also 

 described by Krause), from necrotic masses in a leucaemic spleen. 

 Colonies only visible at end of forty-eight hours ; stroke cultivations, like 

 those of No. I, fatal to mice and rabbits in about four days. Symptoms at 

 first like those obtained with i and 2, but soon followed by suppuration 

 and general infection. 



