420 APPENDIX. 



hut to little other disturbance. Like the Staphylococcus pyogenes albus 

 appears to be associated with suppurative processes and is found under 

 similar conditions. 



(2) Staphylococcus pyogenes citreus. — Found in the pus of acute abscesses ; 

 differs from No. i only in the fact that instead of being dark orange yellow 

 it remains bright citron yellow in colour. 



(3) Diplococcus subflavus. — Grows rapidly on nutrient jelly and blood 

 serum, first as whitish points which gradually become yellowish and then 

 deep yellow ; in large quantities produces abscesses ; occurs in several 

 secretions as a diplococcus from 0.5 to 1.5/1 in diameter ; it is made up of 

 two hemispheres with a central division and resembles the gonococcus 

 somewhat in appearance, but retains the aniline dyes much more tenaciously 

 than that organism. 



(4) Streptococcus coU gracilis. — Occurs in the intestinal canal and faeces 

 of the carnivora ; on plates it forms small sharply qutlined dark colonies 

 in the centre of an area of clear liquefied gelatine ; these, later, become 

 somewhat crenated at the margins ; in a gelatine tube the medium is 

 liquefied rapidly along the track of the puncture and after six or eight days 

 there is precipitated a white finely granular mass ; on agar-agar, potatoes 

 and blood serum there is very little superficial growth even at the body 

 temperature, which is most favourable to its growth ; it is a coccus from 

 .2 to ,4/t in diameter ; in fresh gelatine cultures it forms curved chains 

 consisting of from six to twenty cocci. 



/3 The colonies are found occupying both the centre and periphery of 

 the liquefying area, 

 (i) Micrococcus coronatus. — Appears on the second day in plate cultivations 

 as whitish yellow points ; deep colonies, under the microscope appear as 

 opaque sharply-defined plates ; superficial growths project slightly, this is made 

 more marked by a slight zone of depression surrounding the gelatine ; at 

 intervals tooth-shaped processes advance beyond the general circular peri- 

 phery ; the older growths are dark in colour, newer growths are yellow or 

 yellowish brown ; liquefaction takes place around the growth in presence 

 of air; the coccus, 1. 1 to i.2/< in diameter, occurs singly in short chains or 

 in irregular masses. 



(2) Micrococcus radiatus. — Growths visible in twenty-four hours ; I mm. in 

 diameter in two days ; white or yellowish green, sharply defined, granular, 

 or with outgrowths like the rays of a star fish ; 9olonies sink as gelatine be- 

 comes liquid, and a series of circles of rays formed of delicate threads project 

 radially, this zone increasing in breadth towards the periphery ; one, two or 

 three of these circles are seen according to the age of the growth, the 

 rays of the outer circles always being shorter than those- of the inner ones, 

 each circle forms in about two days; in a puncture cultivation isolated 

 points form along the track of the needle ; from these, lateral branches 

 project ; a funnel-shaped area of liquefaction is formed very slowly, it 

 extends for a short distance only into the gelatine ; micrococci .8 to .9/1 in 

 diameter ; usually grouped in small masses but sometimes in short chains. 



(3) Micrococcus Jiavus liquefaciens. — Occurs on gelatine plates as small 

 yellow circular, oval, or irregular finely-toothed colonies ; superficial colonies 

 distinctly yellow, cause liquefaction. Smaller colonies are found at the 

 border of the liquefying area which has a very sharp outline ; lines of cocci 

 radially disposed run from the centre to the periphery in the clear liquefying 

 area, giving an appearance that is said to resemble the wheel of a waggon ; 



