422, APPENDIX. 



the body, «>., it does not grow under ordinary conditions, 

 see p. 437. 



I. The nutrient gelatine is not liquefied. 



A. Colonies white, nutrient gelatine near growth not 

 stained. 



B. Colonies colourless, nutrient substratum near growth 

 stained, see p. 427. 



c. Colonies cream coloured, see p. 428. 

 D. Colonies of a yellow colour, see p. 428. 



A. Colonies white, nutrient gelatine near growth not stained. 



a. Colonies form minute small translucent drops on 

 plates, delicate growths in stroke and puncture cultiva- 

 tions. 



b. Colonies form thin films on plates, and on the sur- 

 face of tube cultures, see p. 423. 



c. Colonies form white nail-head projections on plates, 

 and nail-shaped growths in tube cultures, see p. 425. 



d. Colonies are branched, not circumscribed, see p. 426. 



a. Colonies form minute small translucent drops on plates, 

 delicate growths in stroke and puncture cultivations. 



(i) Bacillus cholera gailinarum (Fowl cholera). — Grows on gelatine 

 as small, round, white, superficial, finely-granular colonies, light yellow in 

 the centre, a dark zone further out, outlines irregular ; on potatoes do 

 not grow at the ordinary temperature of the room, but at 37° C. grow 

 slowly as yellowish grey transparent drops. Under the microscope average 

 1.2 to 1.5/X in length, and are seen as short rods with rounded ends, 

 which are always more deeply stained with aniline colours at the ends than 

 in the middle, so that they appear like diplococci. Fatal to fowls in from 

 24 to 36 hours, also to mice and rabbits (probably identical with Koch's 

 bacillus of rabbit septicaemia). Not fatal to guinea-pigs, sheep, and horses, 

 but causes absce=s formation. 



(2) Bacillus (Bacteriutn)der Wiliiseudie (Described by Kitl and Hueppe). 

 — Grows on plate cu Itivalions as white or greyish- white colonies, about the size 

 of a pin head, which under the microscope appear to be slightly granular j in 

 the needle track in puncture cultures we see small isolated colonies which 

 run together to form a greyish-white line ; on the surface there are small, 

 white, rounded layers, which grow up from the surface ; on agar-agar they 

 have much the same appearance, but are greyer and more transparent ; on 

 potatoes forms greyish yellow, slightly prominent layers; on blood serum 

 It has a peculiar iritlescent appearance ; grows best at the temperature of the 

 body ; occurs as short rods two to three times as long as they are broad', 

 with somewhat rounded ends; sometimes appear as cocci, or may be 

 ellipsoidal ; Arthrospores are said to be present. Said to be the cause of 

 certain forms of infective pneumonia. It gives rise to most marked symptoms 

 in a number of animals, and is classed by Hueppe with the organisms of 



