43^ APPENDIX. 



broth, flora these smaller masses break off and float in the fluid ; the organism 

 occurs as long threads consisting of bacilli, these bacilli of various lengths 

 are usually of the same thickness, and are frequently very much bent, or 

 curved, or even intertwined ; causes death of rabbits in about eight days, 

 giving rise to a peculiar necrotic process at the seat of inoculation ; kills 

 white mice in about six days. 



(7) Bacillus ofpsuedo septicemia of mice, — Described by Bienstock. An 

 organism found in fieces. Grows on agar-agar very slowly, forming a 

 scarcely visible layer on each side of the inoculation stroke ; is a non-motile 

 organism very like the bacillus of mouse septicaemia, but is somewhat 

 thicker, being sometimes half as broad as long; is occasionally mis- 

 taken for a micrococcus ; found mostly in the cedematous fluid and not in 

 the blood of animals that are inoculated ; kills both rabbits and mice. 



(8) Bacillus of conjunctivitis. — Has been described by Weeks as obtained 

 from the conjunctival sac in cases of conjunctivitis ; will not grow on 

 gelatine, but in .5 per cent, of agar-agar it -grows slowly on plates, form- 

 ing in about forty-eight hours small pearly growths at the point of inocula- 

 tion ; about the fifth or seventh day the growth is complete, and at the end 

 of a month the organisms appear to have lost their power of growth ; it 

 grows exceedingly well in fluid flesh broths, but not in solid media ; grows 

 best at from 34° to 37° C; a bacillus from i/i to 2/» in length and .25^ in 

 thickness ; like the tubercle bacillus, often forms long threads. As 

 another organism was present with this when inoculations into the human 

 subject were made it is not yet quite proved that this organism is the actual 

 cause of conjunctivitis. 



(9) Xerose bacillus. — From the xerotic masses of the conjunctiva of a 

 child suffering from keratomalacia, conjunctivitis and hypersecretion of the 

 Meibomian glands. This organism does not grow on gelatine or potatoes, 

 but grows at the temperature of the body on blood serum, and afterwards 

 (though not primarily) on agar ; on blood serum it forms fine dull grey 

 streaks along each side of a surface needle track ; is sometimes floated off 

 by the water of condensation ; is a bacillus about the length of the mouse 

 septicsemia bacillus ; usually collected into little groups ; a peculiar fatty 

 substance forms a kind of capsule which makes it appear to vary in thick- 

 ness according to the method of staining used. 



(10) Bacillus der Akne contagiosa des Pferdes (Bacillus of horse-pox). — 

 Grows best on blood serum at 37° C. ; on the surface along the stroke track 

 of the needle grows as white points, which later become yellowish'grey, 

 some of this growth is washed away by the condensation water, but sinks 

 to the bottom leaving the watey quite clear ; also grows in gelatine as small 

 white round points, which gradually increase in size, and on gelatine as 

 minute strongly refractile points; small rods 2/« in diameter, usually straight 

 or slightly curved ; when injected subcutaneously the bacillus gives rise to 

 the so-called acne in the horse, calf, sheep, and dogs ; causes erysipelatous 

 swelling and pus formation in the rabbit ; when injected subcutaneously is 

 fatal to guinea-pigs and mice. 



IV. Organisms will only grow under conditions of anaero- 

 biosis. 



(i) Bacillus of malignant cedema {Bac. odematis nmligni, vikrion 

 eptigue).—Gio-wi deep down in gelatine as small " bubbles " with fluid 



