338 UTRICULARIA NEGLECTA. [Cuar. XVII. 
again after 1 hr. 30 m., most of them presented a somewhat different 
appearance. A third specimen was treated with a weaker solution 
of one part of the carbonate to 487 of water, and after 1 hr. the glands 
were pale brown and contained numerous granules. 
Four summits were irrigated with a solution of one part of nitrate 
of ammonia to 437 of water. One was examined after 15 m., and the 
glands seemed affected; after 1 hr. 10 m. there was a greater change, 
and the primordial utricles in most of them were somewhat shrunk, 
and included many granules. In the second specimen, the primordial 
utricles were considerably shrunk and brownish after 2 hrs. Similar 
effects were observed in the two other specimens, but these were not 
examined until 21 hrs. had elapsed. The nuclei of many of the 
glands apparently had increased in size. Five bladders on a branch, 
which had been kept for a long time in moderately pure water, were 
cut off and examined, and their glands found very little modified. 
The remainder of this branch was placed in the solution of the nitrate, 
and after 21 hrs. two bladders were examined, and all their glands 
were brownish, with their primordial utricles somewhat shrunk and 
finely granular. 
The summit of another bladder, the glands of which were in a 
beautifully clear condition, was irrigated with a few drops of a mixed 
solution of nitrate and phospate of ammonia, each of one part to 437 
of water. After 2 hrs. some few of the glands were brownish. After 
8 hrs. almost all the oblong glands were brown and much more opaque 
than they were before; their primordial-utricles were somewhat shrunk 
and contained a little aggregated granular matter. The spherical 
glands were still white, but their utricles were broken up into three or 
four small hyaline spheres, with an irregularly contracted mass in the 
middle of the basal part. These smaller spheres changed their forms 
in the course of a few hours, and some of them disappeared. By the 
next morning, after 23 hrs. 30 m., they had all disappeared, and the 
glands were \brown; their utricles now formed a globular shrunken 
mass in the middle. The utricles of the oblong glands had shrunk 
very little, but their contents were somewhat aggregated. Lastly, the 
summit of a bladder which had been previously irrigated for 21 hrs. 
with a solution of one part of sugar to 218 of water without being 
affected, was treated with the above mixed solution; and after 8 hrs. 
30 m. all the glands became brown, with their primordial utricles 
slightly shrunk. 
Four summits were irrigated with a putrid infusion of raw meat. 
No change in the glands was observable for some hours, but after 
24 hrs, most of them had become brownish, and more opaque and 
granular than they were before. In these specimens, as in those 
irrigated with the salts of ammonia, the nuclei seemed to have 
increased both in size and solidity, but they were not measured. Five 
summits were also irrigated with a fresh infusion of raw meat ; three 
of these were not at all affected in 24 hrs., but the glands of the other 
two had perhaps become more granular. One of the specimens which 
