y^ 



42 



138. Prepare eight test-tubes. Each test-tube is to be 

 half filled with 1% sodium carbonate and 2 cc. of the pan- 

 creatic extract added. To tubes prepared as above add the 

 following : 1, a bit of fibrin ; 2, a small piece of dried 

 albumin ; 3, a piece of meat ; 4, a crumb of bread ; S, a bit of 

 cooked potato ; 6, a bit -of cheese ; 7, a small piece of g-elatin ; 

 8, a small amount of each of the above substances. Keep 

 these in the water bath at 40°C. Note particularly any 

 changes that may occur in No. 1, and compare with the fibrin 

 digested with the gastric juice. In those tubes which first 

 show signs of digestive action, test the contents for alkali- 

 albumin by neutralization. (Similar to the acid-albumin test 

 the only difference being the reaction of the digestive fluid). 

 Test also for albumoses. Place the tubes in the incubator 

 until the next exercise and, after filtering, again test them for 

 alkali-albumin, albumose, and peptone, as with preceding 

 tests. (124-127). Reserve a portion of the contents of tubes 

 No. 1 and 2 for indol test later. (150). 



139. Place a bit of fibrin in two tubes. Half fill the 

 tubes with \^o sodium carbonate. To one tube add 2 cc. of 

 the pancreatic extract. To the other tube add 2 cc. of bile and 

 2 cc. of the pancreatic extract. Let the tubes digest at 40°C. 

 find note in which tube peptone first appears. 



140. Take three test tubes, add 5 cc. of boiled starch 

 mixture and 5 cc. of 1% sodium carbonate to each tube. To 

 No. 1, add 2 cc. of the pancreatic extract. To No. 2, add 2 cc. 

 of bile. To No. 3, add 2 cc. of bile and 2 cc. of the pancre- 

 atic extract. Place the three test tubes in the water bath at 

 40°C. Test a few drops from each tube every minute upon a 

 white plate with a drop or two of iodine and note in which 

 tube the starch first disappears. 



141. Prepare two tubes for starch digestion as follows : 

 In one place 5 cc. of 2% boiled starch and 5 cc. of 1% sodium 

 carbonate and 2 cc. of pancreatic extract. la the other place 

 1 gram of unboiled starch triturated in 5 cc. of cold water, add 

 5 cc. of 1% sodium carbonate and 2 cc. of pancreatic extract. 

 Place both tubes in the water bath at 40°C. and test at inter- 

 vals as in No. 140. Continue the digestion long enough to 

 find sugar in both tubes by the Fehling's test. 



