' BuBBATJ OF Ageicultueb. 343 



the mallein injected subcutaneously. Beginning eight or 

 nine hours after the injection, the temperature should be 

 recorded every two hours for eight or ten hours. The 

 difference in the temperature before and after the in- 

 jection determines whether or not the animal is infected. 

 Two degrees Fahr. is considered suspicious and two and 

 a half or three degrees is considered a re-action. The 

 other and by far the most satisfactory method, because 

 less time is required and just as reliable, is called the 

 opthalmic test. This test is made by the instilling into 

 the conjunctival sac of the healthy eye one or two drops 

 of crude mallein. If the animal is infected, the eyelids 

 will swell and in eight or ten hours afterwards pus will 

 be discharging from the eye, which indicates infection. 



There are other tests, which are slightly more ac- 

 curate and reliable than either of the above, but a labora- 

 tory and special preparations are necessary. The ag- 

 glutination test, the complement fixation test, and the 

 precipitin re-action are gaining more favor because there 

 are more laboratories available now than ever before. 

 However, the subcutaneous or the opthalmic tests are sat- 

 isfactory. 



"We have been very fortunate during the past year, 

 as only one known outbreak of glanders has occurred in 

 this state, and that was on a farm in Jessamine county, 

 where one mare, a standard bred trotting mare, was 

 showing clinical symptoms of nasal glanders, but as no 

 diagnostic symptoms were manifested, the opthalmic test 

 was, made with a typical re-action. The complement fixa- 

 tion test was also made to conform the diagnosis. The re- 

 sult of this test was just as was expected and confirmed 

 the diagnosis made by the opthalmic test. The animal was 

 destroyed. All exposed animals were tested, but only 

 one re-actor was found, and this one was immediately 

 destroyed. The stalls where these animals had been kept 

 were thoroughly cleaned by burning all loose lumber and 

 washing that remaining with a 5% solution of Cresol 

 Compound. All harness was soaked in a strong disin- 

 fectant solution before it was used by other animals. 

 Stable manure, etc., containing the secretions from these 

 animals was burned. No other suspected cases have been 

 reppFliecl frpm that community. 



