22 



The whole mass of "bread, vshich meastired eight, inches in length and 

 one inch in thiclmess, was permeated by the fungus. Labyrinth- like 

 passages were formed in abxmdance and their surfaces were covered with 

 conidia. In no cultures were there any sclerotia formed, even when 

 Irept for over six months. In his attempts to produce sclerotia the 

 only thing Brefeld v/as able to obtain was a visible coloring of the 

 fungous growth to violet, as observed in ergot sclerotia, but careful 

 investigations showed that this was not to be considered in any way 

 as indicating formation of sclerotia. 



Engelke (62) grew C. -purpurea on two different artificial 

 media with the same results as Brefeld. He used nutritive solutions 

 and hard media. The first consisted of 5 percent glucose, 0.5 percent 

 ammonium nitrate, 0.25 percent primary potassium phosphate, and 0.125 

 percent magnesium sulphate. For nutritive hard media, he added 2 per 

 cent agar-agar to the above. He also used ascospores of C.. -pxtrpurea . 

 Tlie fungus grew well on both media, but in the nutritive solution the 

 groivth was somewhat better. In solid media it is more inclined to pass 

 into what he called "Micro sclerotia" which is especially favored by 



the lowering of the temperature. He kept the temperature between 

 15-25°C. Also in a solution containing 10 percent glucose and 5 percent 

 crystal sugar (proJiably cine sugar) the fungus shov^efi equally as good 



growth, vAiile in 5 percent lactose it showed a weaker development of 



conidia, Ho gas bubbles were observed in any of the nutritive solutions 



used. 



Brown and Ranck (33) grew C. pas-pali on bean pods. The 



fungus grev/ slowly throughout the pod, but did not show aiy signs of 



producing reporductive Jjodies. 



