MORBID ANATOMY 6 1 



Bacteriological notes. At the autopsy an agar tube was inoculated 

 with a platinum loop lightly rubbed over the pleural exudate. On the 

 following day a thin grayish growth with condensation water clouded. 

 Examination of hanging drop and stained coverglass preparations shows 

 only swine-plague germs. 



In coverglass preparations of hepatized lung tissue a large number 

 of germs resembling swine-plague bacteria were seen. 



With a bit of hepatized lung tissue, a rabbit was inoculated subcu- 

 taneously at 2 p. M. The rabbit was dead next morning at 8 A. Ri., i. e., 

 in less than i8 hours. In spleen, liver, and blood preparations numer- 

 ous polar-stained swine-plague germs present. An agar culture from 

 heart's blood contained only swine-plague germs. 



From the peritoneal exudate of pig, consisting of cells and fibrin 

 and numerous bacteria of several varieties, two agar plate cultures were 

 made. On Plate A one large colony of spore-bearing bacilli and one 

 small colony of swine-plague germs. Plate B, completelj- overgrown by 

 the spore-bearing bacillus. 



A bouillon culture from the exudate contains streptococci and 

 swine-plague bacteria. Agar plate cultures were made therefrom and 

 both germs isolated. . 



At the same time a large rabbit was inoculated subcutaneously with 

 a bit of the exudate Dead in iS hours. Stained coverglass prepara- 

 tions of blood from heart, spleen, and liver tissue show polar-stained 

 swine-plague germs. An agar culture from the blood contains onlj' 

 swine-plague germs. 



From the spleen, after thoroughly scorching through the exudate, 

 two agar plate cultures and a bouillon culture were made with bits of 

 spleen pulp. The bouillon culture contained only swine-plague germs. 



The swine-plague bacteria were thus obtained from lungs, pleura, 

 spleen, and peritoneal exudate, while hog-cholera bacilli, which were 

 looked for with the greatest care, could not be found." 



§ 50. Differential diagnosis. In sporadic cases, swine 

 plague is to be differentiated from broncho-pneumonia due 

 to other causes than the swine-plague bacterium. Pneumonia 

 of a non-specific nature is often associated wntli deaths due to 

 dietarj' or other causes. 



In epizootics or outbreaks it is to be distingui.shed from 

 hog cholera when there is accompan^-ing catarrhal pneumonia. 

 To make a po.sitive diagnosis it is usually necessary to depend 

 upon the results of the bacteriological examination. 



Pneumonia resulting from lung worms, (Strongylus para- 

 doxus) can be distinguished by a careful examination of the 

 contents of the bronchioles. 



Digitized by Microsoft® 



