162 Modern Microscopy 



solution of acetic acid in distilled water until they are of a 

 pale-blue colour ; watch carefully, or too much colour may 

 be removed ; they are then rinsed in lithia water (1 in 70) 

 1 c.c, water 34 c.c, and transferred to water. The sections 

 are now to be taken up one at a time on the point of a 

 needle and dipped into absolute alcohol, in which some 

 methylene blue has been dissolved. Dehydrate in methylene 

 aniline oil, made as follows : Eub up about 10 grammes of 

 methylene blue with 10 c.c. of aniline, and let the mixture 

 settle. When dehydrated, rinse in aniline, and place for a 

 few minutes in terebene to clear, then mount in Canada 

 balsam. 



Schutz's Method. — Stain the sections or cover-glass 

 films in methylene blue, 1 gramme ; rectified spirit, 

 20 c.c. ; distilled water, 80 c.c, for several hours. Wash 

 in a I per cent, solution of acetic acid, dehydrate in ab- 

 solute alcohol, and clear in cedar-oil, and mount in Canada 

 balsam. 



Syphilis Bacillus. — Stain by Lusgarten's method as 

 follows : 



Aniline-oil ... ... ... ... ... 3 c.c. 



Distilled water ... ... 100 c.c. 



Saturated alcohol solution of gentian violet 11 c.c. 



Alcohol 10 c.c. 



Sections or cover-glasses are placed in the above for from 

 twelve to twenty-four hours. They are then transferred 

 to absolute alcohol for a few minutes ; then place for ten 

 seconds in a 1 per cent, solution of permanganate of 

 potassium, and wash in 5 per cent, solution of sulphuric 

 acid to decolourize the ground tissue. Wash in water, 

 dehydrate in absolute alcohol, clear in cedar-oil, and 

 mount in Canada balsam. 



Bacillus of Enteric Fever — Gaffky's Method. — Sec- 

 tions or cover-glasses are placed for twenty-four hours in 

 a strong solution freshly made by adding a saturated alco- 

 holic solution of methylene blue to distilled water. They 



