Staining and Mounting Micro-Organisms 165 



are then washed in distilled water, dehydrated in absolute 

 alcohol, cleared in terebene, and mounted in Canada balsam. 

 Spirillum. — On cover-glasses these are easily stained by 

 any aniline dye solutions. When in sections use th& 

 following stain for twenty-four hours : 



Bismarck brown ... ... ... 1 gramme. 



Eectified alcohol ... ... ... 20 c.c. 



Distilled water 80 c.c. 



Then wash in water, dehydrate in absolute alcohol, and! 

 mount in Canada balsam. 



Actinomycosis. 



Stain the sections in the following for ten minutes, 

 warmed to about 45° C. : Magenta, 2 parts ; aniline-oil, 

 3 parts; rectified spirit, 20 parts ; distilled water, 20 parts. 

 Wash in water. Place in a concentrated alcoholic solution 

 of picric acid for five to ten minutes. Wash in water, 

 dehydrate in alcohol, clear in clove-oil, and mount in Canada 

 balsam. 



Weigert's Method. — Glacial acetic acid, 5 c.c. ; absolute 

 alcohol, 20 c.c. ; distilled water, 40 c.c. ; add orseille until a 

 dark-red fluid is obtained. Stain the sections in the above 

 for an hour ; rinse quickly in alcohol. Clear in cedar-oil 

 and mount in Canada balsam. 



Hsematozoa of Laveran. — Touch a drop of blood with a 

 perfectly clean cover-glass, apply another cover-glass, press 

 them gently together, then slide them apart, and dry. Now 

 stain in an alcoholic solution of methylene blue, wash in 

 water, dry and mount in Canada balsam. 



Filaria. — Specimens are obtained by pricking the finger 

 of the patient and applying a drop or two of blood to a glass 

 slide ; spread evenly with the aid of a thin glass rod, and 

 allow it to dry. Now apply a few drops of Ehrlich's 

 hsematoxylin, and stain for about five minutes. Wash in 

 distilled water, then stain again in a solution of eosine in 

 alcohol, rinse in water, let the slide stand up on end to 



11—2 



