Cutting, Staining, and Mounting Vegetable Sections 171 



Carmine Stain A. — Borax ... ... 10 grains. 



Distilled water ... 1 ounce. 



Glycerine ... ... ^ ounce. 



Alcohol rect. ... \ ounce. 



Dissolve the borax in the water, and add the glycerine 

 and alcohol. 



B. — Carmine ... ... 10 grains. 



Liq. ammoniae ... 20 min. 

 Distilled water ... 30 min. 



Dissolve the carmine in the water and ammonia. Mix 

 A and B together and filter. 



(1) Place the section in green stain for five to ten 

 minutes. 



(2) Wash in water. 



(3) Place in carmine from ten to fifteen minutes. 



(4) Wash well in methylated spirit. 



(5) Dehydrate and clear in clove-oil. Wash in turpen- 

 tine and mount in Canada balsam. 



Double Staining— M. J. Cole's Method. 



Pure carmine ... ... ... 1 dram. 



Liq. ammonia ... ... ... 2 drains. 



Dissolve the carmine in the ammonia and add 12 ounces 

 of a saturated solution of borax in distilled water. Filter 

 through paper and keep in a stoppered bottle. 



Bleach the sections, and after being well washed with 

 repeated changes of water they are placed in the above 

 stain for five to ten minutes. Then wash well in methylated 

 spirit, and soak in acidulated alcohol — 1 part hydrochloric 

 acid to 5 of methylated spirit — until the excess of stain is 

 removed ; about two minutes is usually sufficient. Wash again 

 in methylated spirit to remove all trace of acid. Dissolve 

 5 grams of acid aniline green in 6 ounces of methylated 

 spirit, and filter if necessary. Soak the section in this green 



