714 



Laura Florence 



TECHNIQUE 

 In the laboratory the following methods of keeping lice have been tried: 



Opportunities j ,, 



Conditions Temperature of feeding in , ,?, 



24 hours °' '"^ 



On laboratory table in petri Room 4 7-10 daj^s 



dishes temperature 



In incubator in open vials 36°-37° C, 4 Under 24 



dry heat hours 



In incubator in vials having in 36°-37° C, 4 3 daj's 



the bottom a layer of moist moist heat 

 cotton batting |-| inch thick 

 In a gauze bag worn on the body 35° C.± Constant Lice would 



not feed 

 through 

 gauze 

 In vials plugged with cotton and 35° C.± 3-4 Up to 35 days 



gauze and carried close to the 

 body day and night 

 As in last experiment 35° C.d= First claj% 3; 26 days 



second to 

 fifteenth 

 day, 2; 

 sixteenth 

 to twenty- 

 sixth da}-, 1 



We have foiuid the last method the best for rearing hog lice in captivit,v. 

 In the glass containers, hog bristles and teased gauze were pro^aded as a 

 foothold. 



Chloroform has been found the most satisfactory medium for killing 

 lice both for dissection in toio and for sectioning. For the former purpose, 

 the lice may be preserved indefinitely in 80- to 85-per-cent alcohol, but 

 the following fluid has been found much more satisfactory: 



Distilled water 30 parts 



Alcohol, 96-per cent 15 parts 



Formaldehyde 6 parts 



Glacial acetic acid 4 parts 



