Mycotic Lymphangitis : Epizootic Lymphangitis. 297 



envelope of each (Nocard). It stains easily by the Gram-NicoUe 

 method : Take on a cover-glass, a smear of pus from the wound, 

 ulcer or pustule, dry, pass three times through the flame, stain 

 five minutes in NicoUe's violet (saturated solution of gentian violet 

 in 90% alcohol 10 cc ; 1% aqueous solution of carbolic acid 

 loocc), run it off and wave for a moment or two in water, cover 

 for 3 or 4 minutes with Gram's solution (I. i part, KI. 2 parts, 

 distilled water 200 parts), run this off, treat with alcohol for i 

 or 2 seconds, then apply counterstain of vesuvine saturated solu- 

 tion for 3 minutes, run it off, dry, and examine, or mount in 

 Canada balsam, (Pallin). 



Cultures have been made in peptonized bouillon, on agar, 

 nutrient gelatine, on potato, or on horse serum containing 2 per 

 cent, of agar. 



The infection attacks horses and according to Tokishige, 

 cattle, entering by sores and abrasions of the skin of the 

 limbs, or under the harness (back, breast, rump, head). Con- 

 fined at first to the point of infection it becomes multiple, be- 

 ing conveyed from place to place, through the soil, floor, stall, 

 harness, blankets, brushes, combs, straw, etc., and many extend on 

 the mucosae. The wounds fester, forming pus and crusts over a 

 more or less considerable swelling. In a variable time, (one to 

 twenty weeks) the surrounding lymphatics become swollen and 

 tender (corded), and nodular swellings appear on their course 

 which fluctuate, burst and discharge a whitish or yellowish pus, 

 sometimes oily or slimy. The disease extends along the lym- 

 phatics, invading the lymph glands and even the internal organs. 



Tokishige describes cases in which it extended along the res- 

 piratory mucosa, causing constriction of the nasal passages and 

 larger bronchi, with dyspnoea, and finally, invaded the lung. 

 In other cases it spread from the prepuce, or scrotum, upward 

 along the tunica vaginalis, spermatic cord and testicle. Caparini 

 and Ferner describe it in the orbit, affecting the lids, nictitans, 

 conjunctiva and adjacent parts, with nodules and abscesses. Maz- 

 zanti describes the case of a filly which died with pea- like nodules 

 on the colon, and ulceration, with dirty, black, purulent centres, 

 and indurated borders. Tokishige describes the affected cattle 

 as showing a vast number of subcutaneous, hard, painless 

 nodules, varying in size from a hazel nut to a walnut and covered 

 Tjy light colored skin. The nodules were isolated and not con- 



