Apoplectiform Septicemia in Chickens. 441 



■day, but recovered after three day's illness. Killed on 21st day, 

 all cultures from heart, spleen and kidney remained sterile. Sub- 

 cutaneous, intra -muscular and feeding experiments failed to pro- 

 duce the disease in ducks. 



A /z^^ow injected with .5 cc. bouillon culture intravenously died 

 on the fourth day with the internal lesions of chickens, and blood 

 extravasations on head and neck. Intra-muscular injections and 

 feeding experiments were fruitless. 



In rabbits, intravenous m^^cMo-as of i to 1.5 cc. led in 24 hours, 

 to temperature of 105.3" F-. without impairment of appetite, or 

 other marked sign of illness, and as a rule the subject was found 

 dead next morning. Intra-abdominal and intrapleural in]ections 

 kill in two to four days, and subcutaneous ones in three days. In 

 addition to the lesions found in chickens, there is often bloody 

 urine, a sanguineous lymph on and beneath the cerebral meninges 

 and in the fourth ventricle, and deep congestion of the cancellated 

 tissue of the vertebrae. Streptococci are abundant in the lesions. 



In mice intra-abdom.inalinjections of .01 cc killed in 48 hours, 

 and subcutaneous injections in 2 to 5 days. The lesions were like 

 those seen in birds, and streptococci were abundant. 



In dogs after intravenous injection there was hyperthermia 

 (103.6° and 104. 1 " F.) and lameness of the right fore shoulder 

 joint, followed in 5 to 8 days by recovery. Feeding on the 

 viscera of the diseased chickens, was followed by anorexia, and 

 vomiting only. 



Guinea pigs and sheep proved refractory. 



Immunization. The inj ection into the wing vein of . 5 to 2 . 5 cc. 

 of bouillon culture, sterilized by heat proved protective to the 

 chickens agafnst inoculations of the virus while the check animals 

 invariably died. 



