Dourine. 57 1 



tached nucleolus, and the membrane prolonged into two actively 

 moving flagella. Third, larger pyriform bodies with chromatin 

 nuclei and nucleoli and the membrane prolonged into one or sev- 

 eral flagella. Fourth, fusiform bodies, thick or delicate, each 

 having a chromatin nucleus and nucleolus, and arranged singly 

 or in groups of two, four, six or more, united together at one 

 end and diverging at the other to form a stellate mass. These 

 last, 20 to 30ft long by r.5 to 2/* broad, may perhaps be the adult 

 form of the parasite from which the small granular or spore 

 forms found in the most recent lesions are derived. The fusi- 

 form outline, the deep staining central mass, with its adjacent 

 nucleolus, and the pointed or flagellate membranous prolonga- 

 tions, more or less motile or undulating, are characteristic features. 



In these morphology and evolutionary forms the trypanosoma of 

 dourine has not been shown to differ from that of surra, nor the 

 nagaua or Tsetse disease, the granule form, the spherical, 

 the club-shaped or pyriform, the fusiform with more or less stel- 

 late grouping, are characteristic of all (I,ydia Rabinowitsch, 

 Kempner, Schneider, Buffard). The distinction is found in the 

 pathogenesis of the two diseases. 



With active cutaneous or mucous lesions, the parasite is usually 

 found abundantly in the blood, sperm, milk, vaginal sjecretions, 

 and the erosions of the vaginal mucosa or penis. During inter- 

 missions, however, and in the absence of local lesions, examina- 

 tion of the blood may fail to detect it, yet its inoculation on a dog 

 will usually produce the affection. It disappears from the blood 

 and tissues with great rapidity after death, so that, to prove suc- 

 cessful, inoculations should be made before death or immediately 

 after. They are ineffective after 48 hours. 



Schneider and Buffard, Nocard and others found the tympano- 

 soma in the blood and exudates of horses, asses and dogs, suf- 

 fering from dourine, and failed to find it, in the same localities, 

 in animals of the same species which were free from dourine. 

 The infected blood, preserved for 24 hours in sealed glass tubes, 

 and then inoculated on two dogs produced characteristic dourine, 

 with the extensive production of trypanosoma in the blood, the 

 destruction of blood globules, and the pathognomonic local lesions. 

 Inoculations of two other dogs, with the same material, at the end 

 of 48 hours produced a slight transient hyperthermia only, 



