590 Veterinary Medicine. 



only in the cardiac capillaries and especially in the kidneys. 

 In some cases the dim remnant of the disintegrated blood globule 

 could still be detected around the parasite. 



lyaveran and NicoUe, examining the blood of Italian ca.ses by 

 fixing and staining, found the two forms, round or oval, and piri- 

 form, and claimed that the first passed into the second by seg- 

 mentation. 



l,ignieres working in Buenos Ayres with the most ample op- 

 portunity as regards fresh material and authority to use it, 

 watched the successive changes in the living organisms, and 

 reached further conclusions. He diluted the blood with a 7 per 

 cent, salt solution, or with ox serum or both, until the globules 

 stood apart in the field. The blood can be kept under observa- 

 tion for days under a cover-glass luted with sterile paraffin, and 

 the changes clearly traced. Securing the blood from a subject 

 having a great abundance of infected globules (usually at the 

 height of the hasmoglobinuria) he found mainly \\x^ piriform para- 

 site intra-globular and free, and in the latter an active whirling 

 motion was kept up by means of the flagellum at its pointed' end. 

 As usually arranged in pairs (gemina), whether inside or outside 

 the globule, they are connected by the flagellum attached to their 

 pointed ends. Careful observation enables one to detect in the 

 pyriform mass a small brightly refrangent point like a nucleus. 

 In this form the piroplasma is 3 to 4/u. in length. 



After 4 or 5 hours, and on toward the 8th, the piroplasma has 

 assumed the round or oval form with a small linear prolongation 

 (flagellum) and shrunken to i to i>^/* in diameter. All the 

 piriform bodies pass into the rounded so that this last is the 

 second stage of their development and not the first as was 

 formerly supposed. The round forms are always present in great 

 numbers in the cortex of the kidney in the second stage of the 

 disease (toward the subsidence of the haemoglobinuria) . The 

 refrangent nucleus is no longer to be seen. 



After one, two or more days there appears in the round para- 

 site a chromatine mass, which breaks up into 2, 3, 4 or 5 smaller 

 chromatic bodies, which lyignieres considers as germs. He has 

 seen no division of the protoplasm, but on the contrary the germs 

 escape, yet remain for a time attached to the outer surface of the 

 parent organism. They show rapid jerking movements. 



