Nagana. Tsetse Fly Disease 643 



most; or when completely dried. Heating to 122° F. for 30 

 minutes sterilized, while at 114.8° F. for half an hour the 

 trypanosoma became nonmotile, but not quite nonvirulent. 



Symptoms. Hyperthermia is marked about the time of the 

 appearance of the infusoria in the blood. (Horse 106.7" ! ^^% 

 104°; rabbit 105.8°), and in the horse there was a paroxysm 

 with each new swarm of the parasite. In one ass intermissions 

 were not observed. In rabbits there was no constant ratio be- 

 tween the temperature and haematozoa, hyperthermia was constant 

 or nearly so. The horse had marked hyperthermia up to death, 

 dogs and bats showed a striking descent even to subnormal, be- 

 fore death. 



Atioemia is a marked feature the red globules being greatly re- 

 duced, and they show a tendency to clump in masses instead of 

 forming rouleaux. The serum added to healthy blood has the 

 same effect on that. Leucocytosis is not constant nor excessive 

 (15,000 to 34,000 per cmm. at the highest). In clotting the 

 blood may form a buffy coat. 



CEdema is common in horse, rabbit, cat and dog especially about 

 the head, legs, belly and genital organs. Rabbits often show 

 swellings, excoriations and sores of the labia, prepuce and penis 

 favoring inoculation by this channel. 



Conjunctivitis is common in cats, dogs, rats, rabbits, often in 

 connection with facial oedema, and corneal opacities and ulcers, 

 and turbidity of the aqueous humor may follow. 



Nasal catarrh often interferes materially with breathing. 



Muscular wasting and debility are prominent phenomena, 

 especially in horse, dog, cat and . rabbit, the loss of weight 

 reaching 20 to 30 per cent. 



Lesions. These are like those of surra. Enlargement of the 

 lymph glands, spleen and liver, firmness, friability and dark 

 color of the spleen ; effusions, petechise and even haemorrhages 

 of the serosse, lungs and stomach, and great atrophy of the mus- 

 cles and adipose tissue are prominent features. The liver is 

 always fatty in rabbits. In the shafts of the long bones, the fat 

 is replaced by red marrow. The bone marrow is sometimes red, 

 at others pale. 



The trypanosomata in the blood vary greatly. In infected rats 

 and mice they appear 3 or 4 days after inoculation, are almost 



