CONTENTS. 



CHAPTER I. 

 General Morphology and Biology. 



PAGE 



Introductory — Terminology — Structure of the bacterial cell- 

 Reproduction of bacteria — Spore formation — Motility — 

 Minuter structure of the bacterial protoplasm — Chemical 

 composition of bacteria — Classification — Food supply — Re- 

 lation of bacteria to moisture, gaseous environment, tempera- 

 ture, and light — Conditions affecting bacterial motility — 

 Effects of bacteria in nature — Methods of bacterial action — 

 Variability among bacteria ..... 1 



CHAPTER II. 



Methods op Cultivation op Bacteria. 



Introductory — Methods of sterilisation — Preparation of culture 

 media — Use of the culture media — Methods of the separation 

 of aerobic organisms — Principles of the culture of anaerobic 

 organisms — • Miscellaneous methods — General laboratory 

 rules ........ 26 



CHAPTER III. 



Microscopic Methods. 



The microscope — Examination of hanging-drop cultures — Film pre- 

 parations—Examination of bacteria in tissues — The cutting 

 of sections — Staining principles— Mordants and decolorisers 



Formulae of stains — Gram's method and its modifications 



Stain for tubercle and other acid-fast bacilli — Staining of 



spores, capsules, and flagella — The Romanowsky stains . 89 



