264 



GONOEKHCEA AND SOFT SOKE 



7 



Fig. 71. — Film preparation of pus from soft 

 chancre, showing Ducrey'a bacillus, chiefly 

 arranged in pairs. Stained with carbol- 

 fuchsin and slightly decolorised, x 1500. 



probably due to the organism having died off. On the whole 



the evidence goes to show 

 that the ordinary bubo 

 associated with soft sore 

 is to be regarded as 

 another lesion produced 

 by Ducrey's bacillus. 

 Sometimes the ordinary 



f*" "~ /-j^S&L. J^^ \ P v °g en i c organisms be- 

 • ** flBpVB^' ftk A come superadded. 



■, ' *^^^JR Q This bacillus takes up 



^b «., imtr^^ ~7 *^ e basic aniline stains 



£| Jg^: %W"' "/ fairly readily, but loses 



the colour very rapidly 

 when a decolorising agent 

 is applied. Accordingly, 

 in film preparations when 

 dehydration is not re- 

 quired, it can be readily 

 stained by most of 

 the ordinary combina- 

 tions, though Lbffler's or 



Kuhne's methylene-blue solutions are preferable, as they do not 



overstain. In sections, 



however, great care must 



be taken in the process 



of dehydration, and the 



aniline-oil method (vide 



p. 98) should be used 



for this purpose, as 



alcohol decolorises the 



organism very readily. 



A little of the methylene- 

 blue or other stain may 



be added with advantage 



to the aniline oil used 



for dehydrating. 



Cultivation. — Al- 

 though for a long period 



of time attempts to 



obtain cultures were un- 

 successful, success has 



been attained within recent years. Benzancon, Griffon, and Le 

 i We are indebted to Dr. Davis for the use of Figs. 71 and 72. 



2*r 



Fig. 72. — Ducrey's bacillus from a 24-hour 

 culture in blood-bouillon. X1500. 1 



