670 



LEISHMANIOSIS 



stain, the characteristic bodies can be readily demonstrated 

 ,(Fig. 198). They are round, oval, or, as Christophers has 

 pointed out, cockle-shell-shaped, and usually 2 '5 to 3 - 5 /* in 

 diameter, though smaller forms occur. The protoplasm stains 

 pink, or sometimes slightly bluish, and contains two bodies 

 taking on the bright red colour of nuclear matter when stained 

 by the Eomanowsky combination. The larger stains less 

 intensely than the smaller, is round, oval, heart-shaped, or 



Fig. 198. — Leishman-Donovan bodies from spleen smear, x 1000. 



bilobed, and lies rather towards the periphery of the body — in 

 the region of the " hinge " in the cockle-shaped individuals. 

 The other chromatin body is usually rod-shaped, and is set 

 perpendicularly or at a tangent to the larger mass, with which 

 only exceptionally it appears to be connected. Usually the 

 protoplasm contains one or two vacuoles. Though in spleen 

 smears many free bodies are seen, the study of sections shows 

 that ordinarily their position is intracellular, — the cells con- 

 taining them being of a large mononuclear type (Fig. 199). The 

 view held is that on their entering the circulation they are 



