BIOLOGICAL THERAPEUTICS 



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A positive reaction consists in a rise of temperature of at least 2.5° 

 F., with the temperature rising to 103° or above. In reacting horses at 

 the point of injection a hot^ painful, edematous swelling develops which 

 may persist for several days. 

 Normal horses may show a slight 

 local swelling which disappears 

 rapidly. 



Ophthalmic Mallein Test. 

 Before the application of 

 the test the eye should be exam- 

 ined and in the presence of in- 

 flammatory conditions the tests 

 should not be made. With a 

 dropper or with the' aid of a 

 camels hair brush, 0.1 to 0.2 mil 

 (3 to 4 drops) of concentrated 

 mallein is placed on the lower 

 eyelid. In case malkin discs are 

 used one disc should be placed 

 into the conjunctival sac. Care 

 should be taken that it does not 

 drop out before being dissolved 

 which usually takes from 30 to 

 50 seconds. In case the animal 

 is affected with glanders a reac- 



Method of Applying the Ophthalraio Test 

 for Glanders With the Collapsible Tube 



An Average Positive Reaction to the Oph 

 thalmio Test for Glanders, Showing 

 the distinct purpulent discharge ,_ _ _ _ 



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tion develops in from six to 

 thirty-six hours in the tested eye. 

 The reaction is manifested by a 

 purulent secretion or discharge 

 v.ith the reddening of the con- 

 junctiva frequently associated 

 with an edematous swelling of 

 the eyelids. The reading should 

 be made in from 18 to 36 hours 

 following the application of the 

 test. 

 Intrapalpebral Mallein Test. 



The intrapalpebral or intra- 

 dermal mallein test is made into 

 the lower eyelid. The mallein 

 used is a 50% dilution of the 

 concentrated mallein with phe- 

 nolized salt solution. 



A fold of the skin of the 

 lower lid is grasped by the fore- 

 finger and the thumb as near the 

 margin as possible. The needle 

 is inserted into the skin parallel 



