39 
23. Compare the two mounts as to changes in the tissues. 
RECORD the results in the form of notes or sketches. 
Pathogenicity studies. The pathogenicity of S. Libertiana and the 
ef ae of the symptoms of the disease may be easily studied as 
ollows :— 
24. Inoculate at the base of stems of beans, sunflower or lettuce 
with an agar-block containing mycelium (from petri-dish cultures). 
Cover inoculum with moist cotton and wrap with strip of tinfoil. 
25. Inoculate roots of carrot or turnip by placing inoculum on 
the surface (injured or uninjured), cover with a bit of wet cotton and place 
the roots in a moist-chamber. : 
26. If living ascospores are available, inoculate some host-plants 
with a suspension of these in water. 
KEEP NOTES on method of inoculation, character of infection-courts, 
length of incubation-period and development of symptoms. 
Reisolate S. Libertiana from. the lesions appearing on inoculated plants. 
REPORT 
1. Drawings and report on inoculation-experiments. 
2. Detail the best method of control of this disease in one of the 
crops it affects, explaining how the life-history of S. Libertiana Fckl. war- 
tants the procedure proposed. 
