SALIVAET CELLS 21 



B. — Metazoa or Mitlticellulae Animals. 



The following examples are cells from these animals, 

 isolated from the body. 



Example 5.— Salivary Cells 



Material. — Einse out the mouth two or three times 

 with water, then collect some fresh saliva by spitting 

 into a test-tube or a watch-glass. Let the glass stand 

 quietly until the air-bubbles rise to the surface of the 

 fluid and a sediment settles. The warm-stage, pipette, 

 dilute iodine, magenta, one per cent, acetic acid, one per 

 cent, acetic acid carmine, and indigo or carmine will be 

 needed during the examination. 



Method of Examination. — With the pipette put a drop 

 of the sediment on the slide and lay the cover-glass in 

 position, with one edge resting on a piece of hair or a 

 scrap of paper. Examine first with the low, then with 

 the high power. In the preparation wili usually be 

 found many colorless flat cells, with irregularly polyg- 

 onal outlines and large nuclei. These are epithelium 

 cells from the lining membrane of the mouth, etc., and 

 have nothing to do with the proper salivary cells. It 

 will be weU to examine their shape, size, structure, and 

 the manner in which they are connected. Note also the 

 verj'' large nucleus, which may be made more distinct by 

 staining with magenta or acetic acid carmine. 



MOEPHOLOGY 



a. General Structure. — What is the shape of the cell? 

 Size? Color? Does it have a cell- wall ? Nucleus? 

 If so, what is its position ? Shape ? What does 



