SPOEES OF FUNGI 47 



material over a drop of water on a slide, and by gently 

 tapping the mouldy substance dust some of the spores 

 into the drop. Put on the cover-glass, supporting it at 

 one edge by a piece of hair, and run a drop of fifty per 

 cent, alcohol under the cover -glass, as water alone does 

 not wet the surface of the spores. 



MOEPHOLOGT 



a. General Structure. — To the unaided eye what appear- 

 ance have the spores? Are they numerous or 

 few ? "What color has a mass of spores ? Exam- 

 ine first with a low, then with a high power. 

 What is the shape of the spores? Are they sepa- 

 rate or connected ? Can you distinguish, with or 

 without the use of iodine, acetic acid carmine, 

 magenta, etc., whether or not the cells have wall, 

 protoplasm, chromatophores, nucleus, etc. ? In 

 what respects do these spores resemble yeast 

 cells ? In what respects do they differ from the 

 cells of green slime? How do you account for 

 the wide dispersal of the spores of moulds ? 

 Make drawings to illustrate shape, size, and structure. 



PHYSIOLOGY 



a. Germination. — Dust some spores into a drop of water 

 and with the point of a needle transfer a small 

 portion, so as to get as few spores as possible, to 

 a drop of Pasteur's solution with sugar in a moist 

 chamber, and examine at intervals for several 

 days. Unless great care is taken foreign organ- 

 isms — bacteria, etc. — are likely to interfere with 

 the growth of the spores. The following method 

 of preparation wiU probably give good results : 



