STONEWOET 283 



the first, where have the new internodes formed ? 

 Do you find that new internodes form hetween 

 the others ? If growth is due to the lengthening 

 of the internodes, which have lengthened the 

 most ? Do you find that your results hold good 

 for the branches as well as for the main stem ? 

 Does the diameter of the stem increase also ? 

 Note the liberation of bubbles of oxygen from 

 plants exposed to the sunlight. Compare with 

 Spirogyra. 



i. Movements of protoplasm. — With a high power ex- 

 amine the terminal cells of a leaf of Charq, or, 

 better, an entire internode of Nitella, and note 

 the moving protoplasm. In what part of the cell 

 is it seen? What is the direction of the flow? 

 Examine several cells to see if this is uniform. Is 

 the rate of flow the same in all cells ? Is it uni- 

 form in all parts of the same cell ? Are the chlo- 

 rophyll bodies carried along by the stream? Does 

 the stream flow from one cell into another ? Can 

 moving protoplasm be found in all of the cells of 

 the same plant ? If not, in which cells is it found ? 

 What is the color of the protoplasm ? Note the 

 granules carried along by the current. How long 

 does it take a granule to make the circuit of the 

 cell? 



Examine in like manner some of the rhizoids, 

 and compare the moving protoplasm in them with 

 that in the leaves. 



General Questions. — Is the plant so firmly attached by 

 its roots to the mud in which it grows, and is the root- 

 system so well developed, as to warrant thinking that 

 Chara absorbs a large part of its nourishment through 



