THE FLOWERING PLANT — BUDS 359 



into a saturated solution of picric acid in water, wash 

 the stems for half an hour in thirty per cent, alcohol, 

 then place them in fifty per cent, and seventy per cent, 

 alcohol, each for a day, and keep permanently in eighty- 

 five or ninety per cent, alcohol. 



provide buds of the horse-chestnut, lilac, hickory, 

 tulip-tree, maple, elm, cherry, potato (tuberous portion), 

 'and onion. 



Use will be made of the scalpel, hand-lens, compound 

 microscope, dilute potash, dilute glycerine, Schulze's 

 solution, acetic acid carmine, razor, watch-glasses, and 

 metric scale. 



Method of Examination. — Study first fresh, fully de- 

 veloped buds, then examine the set of preserved speci- 

 mens. If these have been kept in strong alcohol, soak 

 them for two or three hours in a mixture of three parts 

 of fifty per cent, alcohol and one part of glycerine, and 

 keep them moistened with this mixture while the ex- 

 amination is in progress. Section the buds in various 

 directions for the study of both gross and minute 

 anatomy. 



MOEPHOLOGT 



Nahe&eye Characters. 



Examine the buds of the horse-chestnut in 

 spring before they begin to swell, and again when 

 they are partially expanded. 



a. Position. — At what places on the stem are buds 

 formed? Do they occupy a constant position? 

 Do you always find buds in this place? "What 

 determines their position ? How many buds do 

 you find on an internode ? Do they occur singly 

 or in pairs ? 



