362 THE BIOLOGY OF THE PLANT 



Microscopic Characters. 



Make longitudinal sections passing through the 

 middle of the bud, and under the low power note 

 the arrangement of the scales, young leaves, and 

 flowers, if any. Under the high power study the 

 more minute structure of the various parts, the 

 course of the fibro-vascular bundles, the structure 

 and development of the glandular hairs, the ar- 

 rangement of cells at the growing point, etc. 



Make transverse sections and compare with 

 the others. 

 Draw sections of each bud examined. 



E. — Leaves 



Material. — Fresh material is best, but pressed leaves 

 or those preserved in alcohol may be used. To show 

 the changes which take place during the season, particu- 

 larly those which affect cell contents, provide leaves 

 gathered in the spring, in midsummer, and in the au- 

 tumn. 



For the study of the gross anatomy use leafy branches 

 from the morning-glory, violet or pansy, maple, dande- 

 lion, clover, horse-chestnut, elm, bean, locust, grass, pine, 

 honeysuckle, oleander, strawberry. Geranium, Nastur- 

 tium., etc. In the physiological experiments entire plants 

 of the Geranium, Begonia, Fuchsia, corn, primrose, and 

 sensitive plant will be used. 



The apparatus and instruments needed are scalpel, 

 hand-lens, compound microscope, thread, metric scale, 

 razor, Schulze's solution, phloroglucin, acetic acid car- 

 mine, strong alcohol, dilute iodine, vaseline, balance, 

 bell-jar, half a yard of rubber cloth, small thistle tube, 

 tin-foil, watch-glasses, four large tumblers, and two 



