420 UTRICULARIA NEGLECTA. Ouap. XVII 
meat. No change in the glands was observable for some hours, 
but after 24 hrs. most of them had become brownish, and more 
opaque and granular than they were before. In these speci- 
mens, as in those irrigated with the salts of ammonia, the 
nuclei seemed to have increased both in size and solidity, but 
they were not measured. Five summits were also irrigated 
with a fresh infusion of raw meat; three of these were not at 
all affected in 24 hrs., but the glands of the other two had 
perhaps become more granular. One of the specimens which 
was not affected was then irrigated with the mixed solution of 
the nitrate and phosphate of ammonia, and after only 25 m. 
the glands contained from four or five to a dozen granules. 
After six additional hours their primordial utricles were greatly 
shrunk. 
The summit of a bladder was examined, and all the glands 
found colourless, with their primordial utricles not at all 
shrunk; yet many of the oblong glands contained granules just 
resolvable with No. 8 of Hartnack. It was then irrigated with 
a few drops of a solution of one part of urea to 218 of water. 
After 2 hrs. 25 m. the spherical glands were still colourless; 
whilst the oblong and two-armed ones were of a brownish tint, 
and their primordial utricles much shrunk, some containing 
distinctly visible granules. After 9 hrs. some of the spherical 
glands were brownish, and the oblong glands were still more 
changed, but they contained fewer separate granules; their 
nuclei, on the other hand, appeared larger, as if they had 
absorbed the granules. After 23 hrs. all the glands were 
brown, their primordial utricles greatly shrunk, and in many 
cases ruptured. 
A bladder was now experimented on, which was already 
somewhat affected by the surrounding water; for the spherical 
glands, though colourless, had their primordial utricles slightly 
shrunk; and the oblong glands were brownish, with their 
utricles much, but irregularly, shrunk. The summit was 
treated with the solution of urea, but was little affected by it in 
9 hrs.; nevertheless, after 23 hrs. the spherical glands were 
brown, with their utricles more shrunk; several of the other 
glands were still browner, with their utricles contracted into 
irregular little masses. 
Two other summits, with their glands colourless and their 
utricles not shrunk, were treated with the same solution of 
urea. After 5 hrs. many of the glands presented a shade of 
brown, with their utricles slightly shrunk. After 20 hrs. 
40 m. some few of them were quite brown, and contained 
