8 LABORATORY BACTERIOLOGY 



Jan., 1898, p. 77. Smith, The Jour.' of Exper. Med., Vol. IIL (1898), 

 p. 647. 



11. Work for this Exercise. — Make 1000 c.c. of bouillon 

 and distribute it as follows : — 



Put .^e:c. in each of 10 small sterile test tubes. 



Put 300 c.c. in each of 2 large (500 c.c.) Erlenmeyer flasks, 

 and the remainder in a third flask. 



Put •?- c.c. of distilled water in each of 5 small sterile test 

 tubes and sterilize them with the bouillon. (They are to be 

 used subsequently in place of bouillon in making dilutions.) 

 Label the tubes, " Sterile distilled water." All media required 

 to carry out the directions will be furnished by the instructor 

 excepting such as the student is directed to make in Exercises 

 III., IV., and X. 



12. The Preparation of Bouillon. ^ — Take 500 grams of lean 

 beef, remove all fat, and grind it in a sausage machine or have 

 it minced at the butcher shop. Place the minced meat in an 

 agate iron dish and add 1000 c.c. (2 parts water to one of 

 meat) of clear boiled water, cooled to 65° C, and thoroughly stir 

 with a glass irod. Then macerate it with frequent stirring in a 

 water bath at a temperature of 60° C. for i hour after the tem- 

 perature of the meat and water reaches that of the water out- 

 side. Remove the meat by straining the liquid through a piece 

 of cheese cloth. For this a stout iron meat press is desirable. 

 The liquid should equal in quantity the amount of water used ; 

 if it does not, add distilled or clear boiled water to make it 



Association. One of the questions which was carefully discussed at that 

 meeting was that of culture media. The papers and discussions were re- 

 ferred to a committee of nine bacteriologists with instruction to report at a 

 subsequent time. At the meeting of the American PubUc Health Associa- 

 tion in 1S97, 'his committee made its report (printed in January number 

 of its Journal for 1898). It covers very carefully the methods for prepar- 

 ing various culture media which are recommended for standard prepara- 

 tions, thereby tending to insure uniformity in results. This report is a most 

 important publication on bacteriologic technique, " with special reference 

 to greater uniformity in the description and differentiation of species." 



