36 LABORATORY BACTERIOLOGY 



of muscle sugar. To eliminate this, the following method has 

 been recommended.-' Beef infusion is inoculated in the even- 

 ing with a rich fluid culture of some acid producing organism 

 (^B. colt communis) and placed in the incubator. The next 

 morning the white of an egg is added, and the infusion is boiled 

 and filtered. Peptone and salt are added as usual. It is boiled, 

 filtered again, and distributed in tubes or flasks as desired, and 

 sterilized the same as bouillon (12). 



1 SraXh, Journal of Experimental Medicine, Vol. 11. (1897), p. 543. 



