EXAMINATION OF CULTURES 59 



EXERCISE XXIII ' 



EXAMINATION OF CULTURES OF ANAEROBIC BACTERIA 



91. Work for this Exercise. — Examine and carefully de- 

 scribe the appearance of the anaerobic cultures made in Exer- 

 cise XX. 



With the wire loop remove one of the colonies from the 

 depth of the agar culture and examine it microscopically in 

 (a) a hanging-drop preparation, and (3) a stained cover-glass 

 preparation. Stain with carbol fuchsin. Examine microscopi- 

 cally in similar preparations the bacteria from one of the fer- 

 mentation tubes. Describe the appearance of the bacteria in 

 each preparation and make a drawing of a few of them. 



Note the appearance of the cultures inoculated for the study 

 of the gas production. 



Read carefully in the text-books the methods for cultivating 

 anaerobic bacteria. 



