PREPARATION OF CULTURE MEDIA 6/ 



(5^ Small test tubes of bouillon containing 1% grape sugar 



(dextrose, glucose). 

 (5^ Small test tubes of bouillon containing 1% milk sugar 



(lactose). 

 '4s^ Small test tubes of bouillon containing i%,cane sugar 



(saccharose) . 



Group C — (Media favorable for determining the produc- 

 tion of gas.) 



(5 ) Small test tubes of agar containing i ffo grape sugar. 

 (5) Small test tubes of agar containing 1% milk sugar. 

 (5) Small test tubes of agar containing 1% cane sugar. 



Group D. — (Media and tubes favorable for approximate 

 gas analysis and the determining of the aerobic or anaerobic 

 tendencies.) 



(4) Fermentation tubes containing i % grape sugar. 

 (4) Fermentation tubes containing i % milk sugar. 

 (4) Fermentation tubes containing i % cane sugar. 



(The fermentation tubes containing bouillon with sugars may 

 be substituted for the media containing sugars in Groups B and 

 C if desired.) 



Group JS. — (Media either necessary for, or especially desir- 

 able for, the cultivation or differentiation of certain pathogenic 

 bacteria.) 



Acid' agar (put in small test tubes). 



Acid glycerin agar (put in small test tubes). 



Acid glycerin bouillon (put in small test tubes). 



Blood serum (dog) sohdified at 70-75° C. (ground-glass-capped 



tubes) . 

 Loeffler's blood serum (usually small test tubes) . 



103. The Preparation of Acid Agar. — This is prepared the 

 same as ordinary agar (17) with the omission of the sodium 

 hydrate in the bouillon from which it is made. 



