94 LABORATORY BACTERIOLOGY 



EXERCISE XLVI 



BACTERIUM (BACILLUS) ANTHRACIS {Continued') 



143. Work for this Exercise. — Reexamine all of the cul- 

 tures of Bacterium anthracis and describe any changes in their 

 appearance which may have taken place. 



Examine the agar culture for spores in (i) a hanging-drop 

 preparation and (2) a stained cover-glass preparation. De- 

 scribe the appearance of the bacteria and spores in a prepara- 

 tion from each. 



Stain the spores (Exercise XIX.). 



Study ^d describe the appearance of the colonies on the 

 agar plates. Make an outline drawing of a few of the surface 

 and deep colonies. 



^ Reject all cultures except the agar plates, which may be kept 

 until the next exercise for further observation before rejecting. 

 (These cultures should be put in charge of the instructor, who 

 will see that the spores are destroyed before the tubes are 

 cleaned.) 



Inoculate a tube of each medium in Group A and also a 

 tube of Loeffler's blood serum with Bacterium diphtheria from 

 a culture furnished for study at the next exercise. 



References. — Chapters on anthrax in text-books. Chester, 

 Report Delaware College Agric, Exp. Station, July, 1895. Moore, 

 Report N. Y. State Com. of Agr., 1897. Emmerich and Saida, 

 Centralb. f Bakter. u. Parasitenkunde, Bd. XXVIL, S. 776. 



