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PRINCIPLES OF VETERINARY SURGERY 



but contrary to the latter it does not take the Gram stain. 

 CULTURE. — Pasteur has described the method of cul- 

 ture in bouillon, and Liborius and Roux in solid media. It 

 may also be grown in blood and in peritoneal fluid. When 

 the first liquid (blood) is selected it is necessary that it be 

 collected and brought to the incubator in a pipette, if taken 

 immediately after death. The vibrio then grows exuberant- 

 ly, producing numerous bubbles, and disorganizing the blood 

 column. In bouillon it grows slowly at ordinary tempera- 



FiG. 36. 



Blood of a Cavy, Containing the Septic Vibrio in Short Rods and 



Long Filaments. 



tures. On the contrary, it grows rapidly at 37 degrees C. 

 It does not grow except in a vacuum or in an inert gas, hy- 

 drogen, nitrogen or carbonic acid. 



The septic vibrio-bacillus septicus is, in fact, strictly anae- 

 robic. In bouillon at 37 degrees C, the medium becomes 

 cloudy, and after twenty-four hours a trifling quantity of 

 carbonic acid and hydrogen is set free. Gradually the me- 



