PRINCIPLES OF VETERINARY SURGERY 



429 



in fresh bouillons of beef, veal and chicken the bacilli grow 

 quickly. In twenty-fours the media are uniformly cloudy 

 and fine bubbles of gas are set free by agitation. About 

 the fifteenth day germination stops. The culture has the 

 special odor of burning horn or hair, or, more exactly, of 

 ripe cheese. The bouillon always exhibits an alkaline re- 

 action. In the bouillons more than eight days old the spores 

 do not germinate. In five to six days the spores are formed 



Fig. 42. 

 Nicolaier's Bacillus. Old Culture. 



and the bacillus loses its cilia and precipitates to the bot- 

 tom of the vessel. 



A deep stab in a gelatine tube to the depth of ten to 

 twelve centimeters will show nebulous streaks at the end 

 of four to six days in a temperature of i8 to 20 degrees. 

 The streaks, which might be compared with very fine need- 

 les entangled at the center, radiate in every direction to- 

 ward the circumference of the tube. By degrees the gela- 

 tine is liquefied and the colony appears in the form of a 



