650 PRINCIPLES OF VETERINARY SURGERY 



holic solution of gentian violet. The preparations thus ob- 

 tained are usually quite clear, but it may be necessary to 

 prepare several specimens to prove that each one contains 

 the same micro-organism. The microbian agent can be 

 cultivated in bouillon, on gelose, on gelatine, on potato and 

 on carrot. In bouillon the culture is rapid at 38° C. After 

 sixteen hours a marked disturbance of the media is already 

 apparent, and the turbidity accentuates for forty-eight 

 hours, after which the growth seem to stop. The cultures 

 are deposited in the bottom of the flask in the form of an 

 abundant white coating. At no time does the bouillon give 

 an acid reaction. A study of the micro-organism thus cul- 

 tivated reveals a diplobacillus, thick, rather large, round at 

 the extremities and reproducing itself by direct division. It 



♦/ 



Fig. 109. 

 The Same Agent Considerably Enlarged (1200 Diameters J. 



is easily stained with the various aniline stains, especially 

 gentain violet. It is only imperfectly stained by Gram's 

 method. (Fig 109). In the bouillon the micro-organism 

 has but a short life. After three or four days it presents 

 forms of involution somewhat similar to that- of the bacillus 

 of leprosy. On gelose the culture is abundant after twenty- 

 four hours. It is irregular, yellowish and oily in appear- 

 ance. On gelatine the culture is slow and stretches out in 

 the form of greyish white discs with raised edges and pro- 

 jecting centers, and it never liquefies it. On potato the cul- 

 ture is abundant after twenty-four hours and. is composed 

 of a large number of isolated colonies. On carrot the cul- 

 ture is homogeneous, whitish, oily and with a moist surface. 



