214 IMPORTANT VARIETIES OF FISSION-FUNGI. 



udates or fresh portions of the body, the organisms show 

 the usual polar staining with anilin dyes, as in those of 

 septicaemia hsemorrhag. (13, ix). In bouillon there occur 

 streptococcus-like chains (13, x 6). The bacteria are pro- 

 vided with a capsule, but it is not easily rendered visible 

 (Report of the German Pest Commission). In connection 

 with bacteria from a pure culture, we have not often seen 

 them, yet they -may at times be demonstrated by using 

 dilute staining solutions. 



Spontaneous Motility. — Absolutely non-motile, no 

 fiagella. It must be noted that Kitasato observed very 

 sluggish motion, and likewise Kasanski saw movement of 

 the bacteria (C. B. xxiii, 25). Gordon stained, according 

 to the method of van Ermengen, flagella, which are usually 

 single and polar, rarely in pairs and at the sides (C. B. 

 XXII, 170); compare also N. Schultz (C. B. xxiii, 594). 

 According to the statement of the German Pest Commis- 

 sion, what was supposed to be motility was only molecular 

 motion, and the flagella observed may be supposed to be 

 simply precipitated staining materials. 



Staining Properties. — Stain with all anilin dyes. In 

 preparations from pure cultures, the polar staining is not 

 clearly observed. Not stained by Gram's method. In 

 opposition to Kitasato, the statement is made that the 

 bacteria in the blood stain b}' Gram's method. According 

 to Kasanski, the polar staining succeeds especially in blood 

 and old pus. 



Relation to Oxygen. — The bacteria are obligate 

 aerobes. Growth is stopped by the exclusion of oxj^gen. 



Dependence upon Temperature. — Optimum 37°, 

 but it also grows very well at 22°. 



Intensity of Growth. — On all nutrient media, toler- 

 ably rapid. After two or three days a luxuriant deposit 

 is observed. In bouillon diluted with three times its 

 quantity of water, the growth is very much slower. In 

 dilutions of 1 : 10 it is almost entirely absent (Report of 

 the German Pest Commission). 



Liquefaction. — Aljsent. 



Spores. — None formed. The vegetative cells die com- 

 pletely at 55°-60°. 



Involution Forms. — Very characteristic and remark- 



