274 IMPORTANT VARIETIES OF FISSION-FUNOL 



they remain white and disappear with the increasing size 

 of the area of liquefaction. Thus the paler zone disap- 

 pears, and the entire liquefied area becomes colored uni- 

 formly gray (21, viii ; 22, in). 



(6) Magnified seventy thnes : Superficial colonies, at first 

 delicate, granular, roundish, with a smooth border ; later 

 the central zone is colored rosy red, is delicately crumbly, 

 and sometimes has a faint suggestion of streaking. The 

 peripheral zone consists of continuous little tufts of hairs, 

 which terminate externally in very fine points (21, yii ; 

 22, iv). Besides this form, there are often atypical ones 

 with a brownish center, the separate zones being lost, and 

 the whole colony appearing covered with extremely deli- 

 cate hairs. One form passes into the other. The deep 

 colonies are uncharacteristic, yellowish-brown, granular, 

 whetstone-shaped. 



Gelatin Stab. — After six hours liquefaction begins 

 at the surface of the gelatin in a saucer shape. The 

 liquefaction extends along the stab canal, forms a tube- 

 or cone-shaped funnel, and continues to possess a funnel 

 form in the advanced stage. Only after a very long 

 time does the liquefaction become cylindric. The funnel 

 of liquefaction is filled with whitish or rose-red flocculi, 

 among which more deeply stained clumps are swimming. 

 When liquefaction has advanced verj- far, a cloudy, red- 

 dish to deep red precipitate is at the bottom and the 

 supernatant fluid remains red. When the culture grows 

 atypically, no red color is seen. The form of the funnel 

 of liquefaction is most variable (21, i; 22, ii). 



Agar Plate. — (a) Natural size: The colonies appear 

 as minute red points even after thirty-six hours. Those 

 lying upon the surface increase in size perceptibly and 

 become colored from rose-red to dark red. Also, uncolored 

 colonies occur together with these. They are irregularly 

 roundish, sometimes lobed, often with alternating paler 

 and darker zones and distinct cloudy center (21, v; 

 22, VI). 



(6) Magnified seventy times : Both the deep and superficial 

 colonies at first are roundish, of irregular form, pale yel- 

 low with smooth border. Later the deep colonies take on 

 a brownish color with a reddish luster, the border remain- 



