278 IMPORTANT VARIETIES OF FISSION- FUNGI. 



Motility. — Active, serpentine motion. We found the 

 flagella to be sometimes peritrichous (3-4, long, tortuous), 

 sometimes polar (1-2) (23, xi and xii). 



Staining Properties. — Stains by Gram's method. 



Growth is moderately rapid, and best at ordinary tem- 

 perature. 



Gelatin Plate. — Natural size: At first, small, yellow 

 points, later violet. If the liquefaction is rapid, then there 

 is a gray saucer-shaped depression with violet, alternating 

 concentric rings (23, vii). Where colonies do not liquefy, 

 or do so late, they appear as lobulated, fringed, shining, 

 yellowish to violet growths (compare 23, viii). Magnified 

 sixty times : In both weakly and actively liquefying colonies, 

 they almost always at first resemble those of the typhoid. 

 When sunken in, the colonies become crumbly, have a 

 streaked peripheral zone consisting of little hairs, and 

 finally disintegrate into crumbly masses (23, viii). Colo- 

 nies which liquefy very late are internally of a darker, 

 yellow, finally bluish color and opaque, with a crumbly 

 structure. 



Gelatin Stab. — In freshly isolated varieties the lique- 

 faction after two or three days is funnel-shaped; and along 

 the stab canal, tube-shaped. The contents of the funnel 

 are grayish-violet with colored fragments (23, i). After 

 longer cultivation (as in our culture, after two years) 

 liquefaction is almost entirely lost. The surface growth 

 now is shining, lobulated, dirty yellow to violet. Only 

 after two to three months is there a very shallow saucer- 

 shaped depression. 



Agar Culture. — Moist, shining, somewhat elevated, of 

 the same color as the colonies upon gelatin. In the plate, 

 when slightly magnified, the colonies resemble those of 

 the Bact. coli, and are yellowish-gray and faintly granular 

 (23, V). 



Potato Culture. — ^\Vavy, somewhat elevated growth, 

 moist, shining, violet to violet-black. We have also 

 observed, in numerous potato cultures, dirty yellow to 

 brownish-green growths, resembling those of Bact. coli 

 and fluorescens (23, x). 



Bouillon. — Faintly or strongly turbid, sometimes pro- 

 vided with a thick, sometimes with a delicate pellicle. In 



