BACTERIUM MUBISEPTIGUM. 301 



days there is a very shallow depression, in which the 

 colony rests as an exceedingly delicate veil; only distin- 

 guishable from the surrounding medium with difficulty 

 (33, v). The plate represents the colonies as too distinct. 



(6) Magnified fifty times : The colony is visible only 

 with a very narrow opening in the diaphragm. It is not 

 without difficulty that one observes the exceedingly slight, 

 delicate, gray growth of homogeneous or finely granular 

 character and differentiated from the surroundings with 

 little sharpness (38, vi). When magnified 90 times, 

 there is seen what suggests a tangle of threads. Other 

 writers — for example, B. Preisz — describe the colonies as 

 somewhat denser; from a homogeneous or matted nucleus 

 there radiate outward ramifying and interlacing threads, 

 which sometimes wind like a corkscrew. 



Gelatin Stab. — After a few days the stab canal repre- 

 sents the structure of an exceedingly delicate fir-tree, with 

 branches of equal length throughout the entire length 

 (33, III), which after a longer time become in part more 

 and more confluent and remain as delicate, transparent 

 clouds in the gelatin. Upon the surface there gradually 

 forms a slight, pointed depression. The atypical anthrax 

 culture (34, v) presents a similar, but very much coarser 

 picture. 



Agar Plate. — (a) Natural size : Small, ver}' insignifi- 

 cant, whitish-gray points, which are barely visible only 

 when examined upon a dark background. 



(6) Magnified fifty times. Superficial: At first gray, 

 delicate, veil-like; later, more brownish or yellowish. 

 The homogeneous structure becomes finely or moderately 

 coarsely granular and sometimes looks not unlike the 

 granulation of a finely granular sarcina. Deep : Round- 

 ish to whetstone-shaped, yellowish, homogeneous (33, 

 vn), with a smooth or granular border. 



Agar Stab. — Similar to that in gelatin, a little less 

 luxuriant. The branches may be entirely absent. Sur- 

 face growth exceedingly delicate, transparent, spreading 

 but little, colorless. Sometimes the growth is indicated 

 only by a little luster (33, iv). 



Agar Streak, — Delicate, exceedingly thin growth (33, 

 II). 



