432 ACTINOMTCETES. 



growth of the true T. B. Especially when young, the 

 cultures are easily mistaken for it. Later the growth be- 

 comes colored somewhat orange to coppery red (63, i) 

 (Rabinowitsch). 



In bouillon cultures a thick, wrinkled pellicle is 

 formed very early. Still, the fluid remains clear, with 

 scarcely any precipitate, indicating aerobic growth. There 

 is a disagreeable ammoniacal odor (Rabinowitsch). In 

 sugar bouillon the pellicle-formation is still more pro- 

 nounced. 



Milk Culture. — Like those of Mycob. lact. a planum. 



Potato Culture. — At first there is a whitish to bright 

 orange growth, with some elevation. After a short time 

 it becomes wrinkled, and in older cultures this is especially 

 prominent, similar to the appearance of glj'cerin-agar 

 streak cultures. The growth is dull, rather dry, scarcely 

 at all glistening. Indol is not formed; according to Rabi- 

 nowitsch, there is a trace. H2S is produced in ordinary 

 bouillon only. Acid production: In 10 c.c. of sugar bouil- 

 lon as much as corresponds to 0.8 c.c. of decinormal 

 sodium hydroxid. There is no liberation of gas, and 

 gelatin is not liquefied. 



The Bacillus friburgensis described by Kom (C. B. xxv, 540) 

 stands about midway between Myc. lacticola a planum and /3 per- 

 rugosum, and, according to our nomenclature, may be called Myco- 

 bacterium lacticola 7 friburgense (Kom) L. andN. Upon glycerin- 

 agar the streak growth at first is white, and more luxuriant than in 

 planum ; later there form sturdy, wrinkled elevations, which in time, 

 especially at room temperature, become coppery red. 



Kom gives the following as characteristic: 



1. Stains by Ziehl-Neelsen's method especially well and is little 

 influenced by acids. 



2. Uniform growth — not interrupted — in gelatin stab. 



3. The surface of the agar culture is depressed in the center, the 

 peripheral zone being elevated. 



4. Upon bouillon it produces a disagreeable but not ammoniacal 

 odor. The characteristics are partly inconstant, partly unessential. 



Inoculation with large quantities of pure culture and diseased 

 organs causes no disease in guinea-pigs, rabbits, chickens, and pigeons. 

 White mice are readily infected by the intraperitoneal injection of 0.5 

 c.c. of a suspension. The animals die in from four to forty days, and 

 present massive nodules in all the abdominal organs, 



