crystals were much larger. The crystals were filtered, washed free of 

 chlorides with water and finally in alcohol and ether and allowed to dry 

 in the air. It was analyzed after drying as above. 



0.6430 gram substance lost 0.0745 gram H 2 0. 



0.S68S gram substance gave 0.0603 gram H 2 and 0.1258 gram C0 2 . 



0.2208 gram substance gave 0.1608 gram BaS0 4 and 0.1252 gram 

 Mg 2 P 2 7 . 



Found: C=6.03; H=1.18; P— 15.80; Ba = 42.85; H 2 = 11.58 

 per cent. 



For heptabarium inosite hexaphosphate (C 6 H 11 24 P (i ) 2 Ba 7 = 2267. 



Calculated: C = 6.35; H = 0.97; P= 16.40; Ba= 42.39 per cent. 



For 16 H 2 calculated: 11.27 per cent. 



With very slight modifications in the method of isolation identical 

 preparations were obtained from oats, corn and from commercial phytin 

 (Anderson 1914). 



In preparing the substance from wheat bran the following method 

 was used : 



The bran, 700 grams, was digested in 5 liters of 1 per cent, hydro- 

 chloric acid for 5 hours. It was then strained through cheesecloth and 

 the liquid filtered. Barium hydroxide (Kahlbaum) was added to the 

 filtrate until the reaction was alkaline. The precipitate was filtered and 

 washed with water and then dissolved in about 3 per cent, hydrochloric 

 acid. The opalescent solution was filtered through charcoal and the 

 filtrate precipitated by adding about an equal volume of alcohol. After 

 standing over night the precipitate was filtered, washed in dilute alcohol 

 and again dissolved in 3 per cent, hydrochloric acid and filtered through 

 charcoal. A dilute solution of barium hydroxide was gradually added 

 to the filtrate until a precipitate began to form. After standing over 

 night the substance had separated out in semi-crystalline form. It was 

 filtered and washed in water, dissolved in 3 per cent, hydrochloric 

 acid and precipitated by alcohol. After filtering and washing with dilute 

 alcohol it was again dissolved in the dilute hydrochloric add and pre- 

 cipitated by barium hydroxide. 



The precipitate was still dark colored and it contained some im- 

 purities, not completely soluble in the dilute hydrochloric acid, apparently 

 of colloidal nature, which could not be removed by filtration. In order 

 to eliminate these impurities the barium precipitate was suspended in 

 water and the barium removed with a slight excess of dilute sulfuric acid. 

 The barium sulfate was filtered off and the filtrate precipitated with 

 excess of copper acetate. The copper precipitate was filtered and washea 

 free of sulfates with water. It was then suspended in water and decom- 

 posed with hydrogen sulfide. After filtering off the copper sulfide a clear 

 and colorless solution of the free acid was obtained. By these various 

 operations the oxalic acid had also been removed, as, after nearly neu- 



27 



