phosphorus was hydrolyzed ; in 16 days two-thirds was hydrolyzed. In 

 35 days the amount of hydrolysis had not altered and after about two 

 years it was again the same. The total and inorganic phosphorus was 

 determined as described by Plimmer and Page. 



The dark-colored solution was filtered and barium hydroxide 

 (Kahlbaum) added in slight excess. After standing over night the 

 precipitate was filtered and washed in water. The filtrate and wash- 

 ings were evaporated on the water-bath and the residue was examined 

 as will be described later. 



The barium precipitate was dissolved in about 2.5 per cent, hydro- 

 chloric acid, filtered, and precipitated by adding about an equal volume 

 of alcohol. The precipitate was filtered and washed in dilute alcohol. 

 The substance was again precipitated four times in the same way. It 

 was then precipitated by barium hydroxide three times from about 2 

 per cent, hydrochloric acid and finally two times more with alcohol 

 from the same strength hydrochloric acid. After the final filtering it 

 was washed free of chlorides in dilute alcohol and then in alcohol and 

 ether and dried in vacuum over sulfuric acid. The substance was then 

 a snow-white, amorphous powder. It weighed 28.4 grams. It was free 

 from chlorides and inorganic phosphate, and bases other than barium 

 could not be detected. 



The substance was then rubbed up in a mortar with 300 cc. of 

 cold water and allowed to stand with occasional shaking for a few 

 hours. It was then filtered and washed in water, alcohol and ether, 

 and dried in vacuum over sulfuric acid. The dry water-insoluble 

 portion weighed 5.4 grams. 



The filtrate from above was neutralized to litmus with barium 

 hydroxide. The precipitate was filtered and washed in water, alcohol 

 and ether, and dried in vacuum over sulfuric acid. It weighed 23.6 

 grams. 



These precipitates were analyzed after drying at 105° in vacuum 

 over phosphorus pentoxide. 



The first, water-insoluble portion gave the following result : 



Found: C==11.46; H=1.93; P=11.59; Ba=39.94 per cent. 



This substance is apparently largely composed of the di-barium 

 inosite triphosphate ; calculated for this, C 6 H 11 O 15 P 3 Ba 2 =690. 



C=10.43; H=1.59; P=13.47; Ba=39.71 per cent. It is, however, 

 not pure but apparently contains some barium inosite di-phosphate 

 because the carbon is high and the phosphorus is low. 



The water-soluble substance which was precipitated with barium 

 hydroxide gave the following : 



Found: C=9.63 ; H=1.63; P=10.91 ; Ba=47.41 per cent. 



This substance also appears to consist largely of the neutral 

 barium salt of inosite triphosphate. Calculated for the latter 



45 



