nitric acid for several hours to a temperature of 75° or higher before 

 appreciable quantities of inorganic phosphorus are split off. 



The phosphomolybdate precipitate was then filtered off and the 

 phosphorus determined as magnesium pyrophosphate in the usual way. 



The digestions were made throughout these experiments at room 

 temperature, about 16° C. 



Ten grams of the bran were digested in 100 cc. of water for the 

 time mentioned and then filtered and 20 cc. used for each determina- 

 tion. The same quantities were used throughout. 



It will be noticed that the hydrolysis is greatest during the first 

 four hours. The percentage of phosphorus represents only that amount 

 which was soluble in the water. 



After digesting in water for 24 hours and then acidifying with 

 hydrochloric acid and shaking for half an hour the following results 

 were obtained : 



Table 3 



CHANGES IN CONDITION OF PHOSPHORUS IN WHEAT BRAN 

 AFTER WATER DIGESTION AND ACIDIFYING 



After digesting the bran for 48 hours in water and then adding 

 100 cc. of 2 per cent, hydrochloric acid and allowing to stand for an- 

 other 24 hours, the following results were obtained : 



The addition of toluol to the water appears to diminish the amount 

 of phosphorus dissolved without affecting the degree of hydrolysis. 



